Production and application of polyclonal and monoclonal antibodies against Spiroplasma eriocheiris

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Production and application of polyclonal and monoclonal antibodies against Spiroplasma eriocheiris

A new species of spiroplasma, Spiroplasma eriocheiris (S. eriocheiris), was identified as a lethal pathogen of tremor disease (TD) in Chinese mitten crab recently. In order to acquire appropriate biological and diagnostic tools for characterizing this newly discovered pathogen, 5 monoclonal antibodies (mAbs) and a polyclonal antibody (pAb) against S. eriocheiris were produced. Among the mAbs, 6...

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Monoclonal and Polyclonal Antibodies Specific to Human Fibromodulin

Background: The unique expression of fibromodulin (FMOD) in patients with chronic lymphocytic leukemia (CLL) has been previously reported. Detecting FMOD in CLL patients using specific anti-FMOD mAbs might provide a promising method in detection, monitoring, and prognosis of CLL. Objectives: In this study, we aimed for producing specific antibodies agains...

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Polyclonal and monoclonal antibodies.

The breadth of repertoire yet beautiful specificity of the antibody response is the key to its physiological efficacy in vivo; it also underpins the attractiveness of antibodies as laboratory and clinical reagents. One aspect of the body's reaction to invasion by a microorganism is the activation and clonal expansion of antigen-reactive B lymphocytes. Once these have matured into plasma cells, ...

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Production, characterization and application of monoclonal antibodies against chicken IgY

Monoclonal antibodies (Mabs) were produced against chicken IgY purified from egg yolks. Purity of the IgY was established using immunoelectrophoresis and reducing and non-reducing polyacrylamide gel electrophoresis. Five stable hybrid clones were characterized and Mabs, all belonging to IgG1 isotype, were found to bind to the IgY heavy chain in Western blots. These clones did not cross-react wi...

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ژورنال

عنوان ژورنال: Scientific Reports

سال: 2015

ISSN: 2045-2322

DOI: 10.1038/srep17871